pAAV-hSyn-SOUL-P2A-tdTomato
(Plasmid #153019)

• Purpose: Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.
• Depositing Lab: Guoping Feng
• Publication: Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1016/j.neuron.2020.06.018.

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Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7061
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOUL
• Alt name: Step-function opsin with ultra-high light sensitivity
• Alt name: ChR2(C128S/D156A/T159C)
• Alt name: SSFO(T159C)
• Insert Size (bp): 930
• Mutation: C128S/D156A/T159C
• Tag / Fusion Protein:
  • P2A-tdTomato (C terminal on insert)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digests

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry