TMPRSS2 g3
(Plasmid #153017)

• Purpose: A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
• Depositing Lab: Jason Sheltzer
• Publication: CRISPR plasmids to knock out SARS-CoV-2 host genes (unpublished)

Backbone

• Vector backbone: Lenti-gRNA-mCherry (Addgene #108099)
• Vector type: Lentiviral, CRISPR
• Selectable markers: mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TMPRSS2 gRNA
• gRNA/shRNA sequence: TTGTTCTTCGAAGTGACCAG
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • mCherry

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry