ACE2 g4 + Cas9
(Plasmid #153014)

• Purpose: A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
• Depositing Lab: Jason Sheltzer
• Publication: CRISPR plasmids to knock out SARS-CoV-2 host genes (unpublished)

Backbone

• Vector backbone: Lenti-Cas9-gRNA-TagBFP2 (Addgene #124774)
• Modifications to backbone: gRNA sequence: TGGATACATTTGGGCAAGTG
• Vector type: Lentiviral
• Selectable markers: TagBFP2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 + ACE2 gRNA
• gRNA/shRNA sequence: TGGATACATTTGGGCAAGTG
• Species: H. sapiens (human)
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Tag / Fusion Protein:
  • TagBFP2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry