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PurposeHigh purity C-to-T base editor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 152998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2T-CMV-BlastR
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2T-CMV-eA3A-BE5-NG-BlastR was a gift from David Liu (Addgene plasmid # 152998 ; http://n2t.net/addgene:152998 ; RRID:Addgene_152998) -
For your References section:
Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. 10.1016/j.cell.2020.05.037 PubMed 32533916