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Addgene

p2T-CMV-EA-BE4max-NG-BlastR
(Plasmid #152992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 152992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2T-CMV-BlastR
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BE4 H47E+S48A-nSpCas9-UGI-UGI
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2T-CMV-EA-BE4max-NG-BlastR was a gift from David Liu (Addgene plasmid # 152992 ; http://n2t.net/addgene:152992 ; RRID:Addgene_152992)

  • For your References section:

    Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. 10.1016/j.cell.2020.05.037 PubMed 32533916
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