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Addgene

pXPE
(Plasmid #15284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP72
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2460
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitous kinesin heavy chain
  • Alt name
    uKHC (KIF5B)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3505
  • Mutation
    The insert is missing the 1st 180 bp of 5' untranslated region of the original cDNA clone
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xmal (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXPE was a gift from Ron Vale (Addgene plasmid # 15284 ; http://n2t.net/addgene:15284 ; RRID:Addgene_15284)

  • For your References section:

    Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells. Navone F, Niclas J, Hom-Booher N, Sparks L, Bernstein HD, McCaffrey G, Vale RD. J Cell Biol. 1992 Jun . 117(6):1263-75. 10.1083/jcb.117.6.1263 PubMed 1607388
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