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TU#708
(Plasmid #15277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    95.77
  • Backbone manufacturer
    Andy Fire (Stanford)
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    czgfp
  • Insert Size (bp)
    350
  • Mutation
    leucine zipper fused to c-terminal half of gfp

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    leucine zipper sequences from Lynne Regan (Yale), vector sequences from Andy Fire (Stanford)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TU#708 was a gift from Martin Chalfie (Addgene plasmid # 15277 ; http://n2t.net/addgene:15277 ; RRID:Addgene_15277)

  • For your References section:

    Combinatorial marking of cells and organelles with reconstituted fluorescent proteins. Zhang S, Ma C, Chalfie M. Cell. 2004 Oct 1. 119(1):137-44. 10.1016/j.cell.2004.09.012 PubMed 15454087
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