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Addgene

pGBW-m4133877
(Plasmid #152686)

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Item Catalog # Description Quantity Price (USD)
Plasmid 152686 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pTwist_CMV_Puro_6
  • Backbone size w/o insert (bp) 6080
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 nsp4
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    1499
  • Mutation
    wt
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cleavable TEV;TRX (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI reference sequence: YP_009725300.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4133877 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 152686)
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