pCAG-NL1(-)
(Plasmid #15260)

• Depositing Lab: Peter Scheiffele
• Publication: Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Backbone

• Vector backbone: pCAAGS
• Backbone size w/o insert (bp): 4921
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neuroligin 1
• Alt name: Nlgn1
• Alt name: Nlgn1
• Species: M. musculus (mouse)
• Insert Size (bp): 2472
• Entrez Gene: Nlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a A43V mutation in the aa acid sequence. The mutation is within the signal sequence and our data demonstrate that cleavage is normal. That means the mature protein does not have any change in amino acid sequence.