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Addgene

pBabe-Puro-RalA-S194A
(Plasmid #15255)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    v-ral simian leukemia viral oncogene homolog A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    621
  • Mutation
    serine 194 to alanine
  • Entrez Gene
    RALA (a.k.a. HINCONS, RAL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer MSCV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Puro-RalA-S194A was a gift from William Hahn (Addgene plasmid # 15255 ; http://n2t.net/addgene:15255 ; RRID:Addgene_15255)

  • For your References section:

    The tumor suppressor PP2A Abeta regulates the RalA GTPase. Sablina AA, Chen W, Arroyo JD, Corral L, Hector M, Bulmer SE, DeCaprio JA, Hahn WC. Cell. 2007 Jun 1. 129(5):969-82. 10.1016/j.cell.2007.03.047 PubMed 17540176
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