GST-RBD
(Plasmid #15247)

• Depositing Lab: Martin Schwartz
• Publication: Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rhotekin
• Species: M. musculus (mouse)
• Insert Size (bp): 249
• Mutation: amino acids 7-89 (Rho binding domain)
• Entrez Gene: Rtkn
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Resource Information

• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry