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Addgene

nAChR alpha4 YFP
(Plasmid #15245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha4-YFP-M
  • Alt name
    nicotinic acetylcholine receptor
  • Alt name
    nAChR alpha4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Entrez Gene
    Chrna4 (a.k.a. Acra-4, Acra4, EBN1, ENFL1)
  • Tag / Fusion Protein
    • YFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jerry Stitzel, Univ of MI, Ann Arbor
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YFP with an upstream HA epitope tag was inserted into BstEII restriction site of the M3-M4 loop at amino acid position 426 (alpha4-YFP-M).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nAChR alpha4 YFP was a gift from Henry Lester (Addgene plasmid # 15245 ; http://n2t.net/addgene:15245 ; RRID:Addgene_15245)

  • For your References section:

    Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed with functional fluorescently labeled subunits: effects of localization, trafficking, and nicotine-induced upregulation in clonal mammalian cells and in cultured midbrain neurons. Nashmi R, Dickinson ME, McKinney S, Jareb M, Labarca C, Fraser SE, Lester HA. J Neurosci. 2003 Dec 17. 23(37):11554-67. PubMed 14684858
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