LB595 (Bcl-2 promoter CRE mut)
(Plasmid #15243)

• Depositing Lab: Linda Boxer
• Publication: Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

Backbone

• Vector backbone: pBSII KS+
• Backbone size w/o insert (bp): 2961
• Vector type: Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: B-cell lymphoma/leukemia-2
• Alt name: Bcl-2
• Species: H. sapiens (human)
• Insert Size (bp): 354
• Mutation: Mutated CRE site at -1537 changed from GTGACGTTA to GGGCCTTTA
• Entrez Gene: BCL2 (a.k.a. Bcl-2, PPP1R50)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Michael Cleary, Stanford University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry