Skip to main content
Addgene

LB334 (Bcl-2 from -1287 to -1640)
(Plasmid #15241)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBSKS+
  • Backbone size w/o insert (bp) 5596
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    B-cell lymphoma/leukemia-2
  • Alt name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    354
  • Mutation
    Bcl-2 fragment from -1287 to -1640 with P1
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site Pst1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LB334 (Bcl-2 from -1287 to -1640) was a gift from Linda Boxer (Addgene plasmid # 15241 ; http://n2t.net/addgene:15241 ; RRID:Addgene_15241)

  • For your References section:

    Induction of bcl-2 expression by phosphorylated CREB proteins during B-cell activation and rescue from apoptosis. Wilson BE, Mochon E, Boxer LM. Mol Cell Biol. 1996 Oct . 16(10):5546-56. PubMed 8816467
Related items:
Commonly requested with: