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Addgene

pWBC
(Plasmid #15239)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS SK-
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human neuronal kinesin heavy chain
  • Alt name
    nKHC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3840
  • GenBank ID
    HSU06698
  • Entrez Gene
    KIF5A (a.k.a. ALS25, D12S1889, MY050, NEIMY, NKHC, SPG10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13Fw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWBC was a gift from Ron Vale (Addgene plasmid # 15239 ; http://n2t.net/addgene:15239 ; RRID:Addgene_15239)

  • For your References section:

    Cloning and localization of a conventional kinesin motor expressed exclusively in neurons. Niclas J, Navone F, Hom-Booher N, Vale RD. Neuron. 1994 May . 12(5):1059-72. 10.1016/0896-6273(94)90314-X PubMed 7514426
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