Alpha 5 integrin-GFP
(Plasmid #15238)

• Depositing Lab: Rick Horwitz
• Publication: Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha 5 integrin
• Alt name: Itga5
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• GenBank ID: X06256
• Entrez Gene: ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: CMV immediate early gene forward primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Louis Reichardt UCSF
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry