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Addgene

Flag-ECFP-betaPIXa
(Plasmid #15235)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-PIXa
  • Alt name
    PAK interactive exchange factor
  • Alt name
    COOL 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1950
  • Entrez Gene
    Arhgef7 (a.k.a. C, Cool, P, PIX, Pak3bp, beta-Pix, betaPi, betaPix, betaPix-b, betaPix-c, coo, cool-1, mKIAA0142, p8, p85Cool1, p85SPR)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Chris Turner SUNY Upstate Medical Univ. Syracuse, NY
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-ECFP-betaPIXa was a gift from Rick Horwitz (Addgene plasmid # 15235 ; http://n2t.net/addgene:15235 ; RRID:Addgene_15235)

  • For your References section:

    Identification of protein networks associated with the PAK1-betaPIX-GIT1-paxillin signaling complex by mass spectrometry. Mayhew MW, Webb DJ, Kovalenko M, Whitmore L, Fox JW, Horwitz AF. J Proteome Res. 2006 Sep . 5(9):2417-23. 10.1021/pr060140t PubMed 16944954
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