Flag-ECFP-GIT1
(Plasmid #15223)

• Depositing Lab: Rick Horwitz
• Publication: Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847-50.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein coupled receptor kinase-interacting protein 1
• Alt name: GIT1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: GIT1 (a.k.a. p95-APP1)
• Tags / Fusion Proteins:
  • flag (N terminal on backbone)
  • ECFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry