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Addgene

pGAL4-CREB
(Plasmid #15221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pM
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREB
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1000
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Tag / Fusion Protein
    • Gal4 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI/SalI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Gal4-N-term primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CREB kindly provided by Richard A. Maurer
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Wild-type CREB fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the cDNA of CREB as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAL4-CREB was a gift from Ugo Moens (Addgene plasmid # 15221 ; http://n2t.net/addgene:15221 ; RRID:Addgene_15221)

  • For your References section:

    The cAMP signalling pathway activates CREB through PKA, p38 and MSK1 in NIH 3T3 cells. Delghandi MP, Johannessen M, Moens U. Cell Signal. 2005 Nov . 17(11):1343-51. 10.1016/j.cellsig.2005.02.003 PubMed 16125054
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