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pET17_K560_GFP_His
(Plasmid #15219)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15219 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET17b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human neuronal kinesin heavy chain
  • Alt name
    K560_GFP
  • Alt name
    KIF5B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET17_K560_GFP_His was a gift from Ron Vale (Addgene plasmid # 15219 ; http://n2t.net/addgene:15219 ; RRID:Addgene_15219)

  • For your References section:

    Microtubule interaction site of the kinesin motor. Woehlke G, Ruby AK, Hart CL, Ly B, Hom-Booher N, Vale RD. Cell. 1997 Jul 25. 90(2):207-16. 10.1016/S0092-8674(00)80329-3 PubMed 9244295
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