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Addgene

Flag-hPIASx beta
(Plasmid #15210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Flag
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIASx beta
  • Alt name
    PIASx
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIAS2 (a.k.a. ARIP3, DIP, MIZ1, PIASX, SIZ2, ZMIZ4)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BamHI/XhoI insert cloned into BamHI/SalI sites of vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-hPIASx beta was a gift from Ke Shuai (Addgene plasmid # 15210 ; http://n2t.net/addgene:15210 ; RRID:Addgene_15210)

  • For your References section:

    PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4. Arora T, Liu B, He H, Kim J, Murphy TL, Murphy KM, Modlin RL, Shuai K. J Biol Chem. 2003 Jun 13. 278(24):21327-30. 10.1074/jbc.C300119200 PubMed 12716907
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