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Addgene

Flag-hPIASy
(Plasmid #15208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Flag
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIASy
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIAS4 (a.k.a. PIAS-gamma, PIASY, Piasg, ZMIZ6)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BglII/XhoI insert cloned into BamHI/SalI sites of vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-hPIASy was a gift from Ke Shuai (Addgene plasmid # 15208 ; http://n2t.net/addgene:15208 ; RRID:Addgene_15208)

  • For your References section:

    A transcriptional corepressor of Stat1 with an essential LXXLL signature motif. Liu B, Gross M, ten Hoeve J, Shuai K. Proc Natl Acad Sci U S A. 2001 Mar 13. 98(6):3203-7. 10.1073/pnas.051489598 PubMed 11248056
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