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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Flag
- Backbone size w/o insert (bp) 3800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePIAS3
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SpeciesM. musculus (mouse)
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MutationV580I
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GenBank IDAAB88902.2
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Entrez GenePias3 (a.k.a. Pias3l)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-mPIAS3 was a gift from Ke Shuai (Addgene plasmid # 15207 ; http://n2t.net/addgene:15207 ; RRID:Addgene_15207) -
For your References section:
Specific inhibition of Stat3 signal transduction by PIAS3. Chung CD, Liao J, Liu B, Rao X, Jay P, Berta P, Shuai K. Science. 1997 Dec 5. 278(5344):1803-5. 10.1126/science.278.5344.1803 PubMed 9388184