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Chx10 3kb AP (CC#318)
(Plasmid #15205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chx10 3kb AP reporter
  • Alt name
    Chx10
  • Alt name
    alkaline phosphatase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3000
  • Entrez Gene
    Vsx2 (a.k.a. Chx10, Hox-10, Hox10, or)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SacII (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 3Kb-AP reporter was constructed using a
genomic BamHI–SacII fragment isolated from a Chx10
BAC (Rowan and Cepko, 2004) and subcloned into pBluescript
(Stratagene). An SV40 intervening sequence (IVS)
was isolated from pNASSbeta (Clontech) digested with EcoRI
and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was
isolated from the SV40all plasmid (gift of S. Dymecki).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Chx10 3kb AP (CC#318) was a gift from Connie Cepko (Addgene plasmid # 15205 ; http://n2t.net/addgene:15205 ; RRID:Addgene_15205)

  • For your References section:

    A POU factor binding site upstream of the Chx10 homeobox gene is required for Chx10 expression in subsets of retinal progenitor cells and bipolar cells. Rowan S, Cepko CL. Dev Biol. 2005 May 15. 281(2):240-55. 10.1016/j.ydbio.2005.02.023 PubMed 15893976
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