pGBW-m4134906
(Plasmid
#151960)
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PurposeMammalian expression plasmid for Human coronavirus OC43 nucleocapsid
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 151960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepTwist_CMV_Puro_6
- Backbone size w/o insert (bp) 6080
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman coronavirus OC43 nucleocapsid protein
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SpeciesHuman coronavirus OC43
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Insert Size (bp)1340
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Mutationwt
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Entrez GeneEYW02_gp8 (a.k.a. EYW02_gp8)
- Promoter CMV
Cloning Information
- Cloning method Unknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
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Articles Citing this Plasmid
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NCBI reference sequence: P33469
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBW-m4134906 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 151960 ; http://n2t.net/addgene:151960 ; RRID:Addgene_151960)