RCAS(B) En cRax deltaC (CC#522)
(Plasmid
#15195)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP (B)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; Avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRax
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SpeciesG. gallus (chicken)
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Entrez GeneRAX2 (a.k.a. RAX, RAXL, RX1, RX2, Rx-like, cRax1, cRaxL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A fusion construct containing the engrailed repressor domain and Rax with deletion only of the oar/paired-tail motif.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS(B) En cRax deltaC (CC#522) was a gift from Connie Cepko (Addgene plasmid # 15195 ; http://n2t.net/addgene:15195 ; RRID:Addgene_15195) -
For your References section:
The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation. Chen CM, Cepko CL. Development. 2002 Dec . 129(23):5363-75. 10.1242/dev.00114 PubMed 12403708