pGBW-m4134897
(Plasmid #151948)

• Purpose: Mammalian expression plasmid for Human coronavirus HKU1 nucleocapsid
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: Human coronavirus mammalian expression constructs (unpublished)

Backbone

• Vector backbone: pTwist_CMV_Puro_6
• Backbone size w/o insert (bp): 6080
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human coronavirus HKU1 nucleocapsid phosphoprotein
• Species: Human coronavirus HKU1
• Insert Size (bp): 1319
• Mutation: wt
• Entrez Gene: N (a.k.a. HCHV1gp7)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI reference sequence: Q5MQC6