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Addgene

pGFP-E p27 (CC#631)
(Plasmid #15192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-MN-IRES-E-GFP
  • Backbone manufacturer
    Gary Nolan, Stanford
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p27
  • Alt name
    Kip1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cdkn1b (a.k.a. Kip1, p27, p27Kip1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • His

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-E p27 (CC#631) was a gift from Connie Cepko (Addgene plasmid # 15192 ; http://n2t.net/addgene:15192 ; RRID:Addgene_15192)

  • For your References section:

    p27Kip1 and p57Kip2 regulate proliferation in distinct retinal progenitor cell populations. Dyer MA, Cepko CL. J Neurosci. 2001 Jun 15. 21(12):4259-71. PubMed 11404411
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