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Addgene

pGBW-m4134900
(Plasmid #151911)

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Item Catalog # Description Quantity Price (USD)
Plasmid 151911 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pTwist_CMV_Puro_6
  • Backbone size w/o insert (bp) 6080
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 spike prefusion-stabilized
  • Alt name
    Spike protein
  • Alt name
    S protein
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3578
  • Mutation
    d1-15 d1209-1273 R682A R685G K986P V987P
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI reference sequence: QHN73810.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4134900 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 151911 ; http://n2t.net/addgene:151911 ; RRID:Addgene_151911)
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