pGBW-m4134909
(Plasmid #151901)

• Purpose: Mammalian expression plasmid for Human coronavirus 229E nucleocapsid
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: Human coronavirus mammalian expression constructs (unpublished)

Backbone

• Vector backbone: pTwist_CMV_Puro_6
• Backbone size w/o insert (bp): 6080
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human coronavirus 229E nucleocapsid protein
• Species: Human coronavirus 229E
• Insert Size (bp): 1163
• Mutation: wt
• Entrez Gene: N (a.k.a. HCoV229Egp7)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI reference sequence: P15130