pCS6/U6
(Plasmid #15173)

• Purpose: (Empty Backbone)
• Depositing Lab: Connie Cepko
• Publication: Harpavat et al BMC Dev Biol. 2006 . 6():2.

Backbone

• Vector backbone: pCMV Sport 6 with U6 promoter
• Backbone size (bp): 5000
• Vector type: Mammalian Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pCS6/U6 vector was made by ligating an M13 PCR product from the pBS/U6 vector (from the Shi lab, Harvard Medical School) to a Rsr II-Mlu I blunted pCMV-Sport6 vector (Invitrogen). This vector is compatible with Invitrogen's Gateway system.

See Author's Map for cloning information and the article for a protocol for cloning hairpins into this vector (in the article's supplementary material).