DAP (DOL-AP) (CC#44)
(Plasmid #15164)

• Depositing Lab: Connie Cepko
• Publication: Fields-Berry et al Proc Natl Acad Sci U S A. 1992 Jan 15. 89(2):693-7.

Backbone

• Vector backbone: pDOL
• Vector type: Mammalian Expression, Retroviral ; Avian expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLAP
• Alt name: placental alkaline phosphatase
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDOL was a gift from Dr. Richard Mulligan. PLAP was a gift from Dr. John Flanagan and Dr. Philip Leder.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Retroviral vector expressing PALP from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.

Plasmid CMV-PLAP was digested with KpnI and a 1930bp fragment encoding PLAP was isolated. The ends of this fragment were blunted with T4 DNA polymerase and ligated to a SalI linker. The linker fragment was digested with SalI and inserted into the SalI site of the Moloney murine leukemia virus retroviral vector pDOL.