RCASBP (E) (CC#36)
(Plasmid #15163)

• Purpose: (Empty Backbone)
• Depositing Lab: Connie Cepko
• Publication: Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Backbone

• Vector backbone: RCASBP (E)
• Backbone size (bp): 12000
• Vector type: Retroviral ; Avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RCASBP(A) provided by S. Hughes.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The name RCAS stands for Replication-Competent ASLV long terminal repeat (LTR) with a Splice acceptor. The RCAS vectors retain the src splice site and express an inserted gene via a spliced message. These vectors will replicate in appropriately chosen avian cells (usually chicken or quail, although others can be used). In some cases, RCAS vectors will infect, but will not replicate, in mammalian cells. Please also see the paper Hughes SH et al., J Virol 1987 Oct; 61(10):3004-3012.

We constructed a fourth vector of the RCAS series of the E subgroup. Two hypervariable regions within the extracellular domain of the env gene are of particular importance in determining the host range of different viral subgroups. Starting with RCASBP(A), a new vector of the E subgroup, called RCASBP(E), was constructed by swapping a portion of the A env with E env. The E env sequences were obtained from pRAV-0 by KpnI-SalI digestion and isolation of the 1.1-kb env fragment. This fragment was ligated to RCASBP(A) in place of the homologous KpnI-SalI fragment.