RCASBP-AP (E) (CC#39)
(Plasmid #15162)

• Depositing Lab: Connie Cepko
• Publication: Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Backbone

• Vector backbone: RCASBP (E)
• Backbone size w/o insert (bp): 12000
• Vector type: Retroviral ; Avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLAP
• Alt name: placental alkaline phosphatase
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RCASBP(A) provided by S. Hughes.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In order to clone PLAP into RCASBP vectors, the Clal2 adaptor plasmid described by Hughes et al. was used to obtain ClaI ends on PLAP. PLAP was removed from pDAP by SalI digestion and inserted into Clal2 at the SalI site. PLAP was removed from Clal2 by digestion with ClaI and inserted into RCASBP(E) at the ClaI site. Orientation of PLAP was checked by polymerase chain reaction amplification with an oligoprimer from the 3' untranslated end of PLAP (5'-GCCACCACCTACAGCCCA GTGG-3').