pLIA CRX EnR (CC#145)
(Plasmid #15155)

• Depositing Lab: Connie Cepko
• Publication: Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Backbone

• Vector backbone: pLIA
• Backbone manufacturer: Cepko Lab
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRX
• Alt name: cone-rod homeobox containing gene
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: amino acids 7–108, containing the CRX homeobox
• Entrez Gene: Crx (a.k.a. Crx1)
• Tag / Fusion Protein:
  • Engrailed Repressor (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI/SnaBI (destroyed during cloning)
• 3′ cloning site: ClaI/SnaBI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For the CRX-EnR fusion construct, a 302 bp XmaI fragment (amino acids 7–108) containing the Crx homeobox was fused to the EnR domain. To make the CRX-EnR fusion, the XmaI Crx fragment, an EcoRI-XmaI linker encoding amino acids 2–6 with a NcoI site at the methionine of position 2, an XmaI-ClaI linker, and a ClaI-NotI (partial digestion) 930 bp fragment of the EnR were ligated into a shuttle vector, pSLAX21. The ClaI site inside this construct was designed so that the entire CRX-EnR fusion could be excised as a 1.3 kb ClaI fragment from pSLAX21/CRX-EnR. The ClaI fragment was ligated into pLIA.