pLIA EnR (CC#29)
(Plasmid
#15152)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLIA
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Backbone manufacturerCepko Lab
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEngrailed repressor
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Alt nameEngrailed
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1000
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Mutationengrailed repressor domain
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Entrez Geneen (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI/SnaBI (destroyed during cloning)
- 3′ cloning site ClaI/SnaBI (destroyed during cloning)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIA EnR (CC#29) was a gift from Connie Cepko (Addgene plasmid # 15152 ; http://n2t.net/addgene:15152 ; RRID:Addgene_15152) -
For your References section:
Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. Furukawa T, Morrow EM, Cepko CL. Cell. 1997 Nov 14. 91(4):531-41. 10.1016/S0092-8674(00)80439-0 PubMed 9390562