BAG (CC#23)
(Plasmid #15148)

• Depositing Lab: Connie Cepko
• Publication: Price et al Proc Natl Acad Sci U S A. 1987 Jan . 84(1):156-60.

Backbone

• Vector backbone: modified pDOL
• Vector type: Mammalian Expression, Retroviral ; Avian expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta-galactosidase
• Alt name: b-galactosidase
• Alt name: LacZ
• Species: Escherichia coli
• Insert Size (bp): 3000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDOL was a gift from Richard Mulligan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Retroviral vector expressing beta-galactosidase from the long-terminal-repeat (LTR) promoter. The neomycin gene is expressed from SV40 promoter.