pRIAS (CC#15)
(Plasmid
#15147)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRIAS
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Backbone manufacturerCepko Lab
- Backbone size (bp) 9795
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- gag (N terminal on backbone)
- pol (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A replication-incompetent avian retroviral vector for transgene expression. Clone your transgene into pSLIRES11 and use ClaI to transfer it to pRIAS.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRIAS (CC#15) was a gift from Connie Cepko (Addgene plasmid # 15147 ; http://n2t.net/addgene:15147 ; RRID:Addgene_15147) -
For your References section:
Production and design of more effective avian replication-incompetent retroviral vectors. Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL. Dev Biol. 1999 Oct 15. 214(2):370-84. 10.1006/dbio.1999.9432 PubMed 10525341
Map uploaded by the depositor.
