pSLIRES-11 (CC#13)
(Plasmid #15145)

• Purpose: (Empty Backbone)
• Depositing Lab: Connie Cepko
• Publication: Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Backbone

• Vector backbone: pSLIRES-11
• Backbone manufacturer: Cepko Lab
• Backbone size (bp): 3552
• Vector type: Adapter vector for cloning into pRISAP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • IRES (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In pSLIRES11, the 11th ATG of the IRES was modified to an NcoI site. The transgene can be introduced into pSLIRES11 by changing its initial methionine codon ATG to an NcoI site. The IRES and the inserted cDNA fragment can be excised as a single fragment using ClaI digestion and can then be cloned into the unique ClaI site of pRISAP. As a result both the PLAP marker and the transgene will be encoded by a spliced bicistronic message. In addition, the gene under the control of the IRES can be translated from the full-length unspliced mRNA.