pRISAP (CC#8)
(Plasmid #15142)

• Purpose: (Empty Backbone)
• Depositing Lab: Connie Cepko
• Publication: Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Backbone

• Vector backbone: pRISAP
• Backbone manufacturer: Cepko Lab
• Backbone size (bp): 11419
• Vector type: Retroviral ; avian expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • gag (N terminal on backbone)
  • pol (N terminal on backbone)
  • PLAP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A replication-incompetent avian retroviral vector expressing the histochemical marker gene PLAP.

Clone your transgene into pSLIRES11 and use ClaI to transfer it to pRISAP. As a result both the PLAP marker and the transgene will be encoded by a spliced bicistronic message.