RCAS GH6 (CC#152)
(Plasmid
#15137)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCASBP (A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; Avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGH6
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SpeciesG. gallus (chicken)
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Entrez GeneHMX1 (a.k.a. GH6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS GH6 (CC#152) was a gift from Connie Cepko (Addgene plasmid # 15137 ; http://n2t.net/addgene:15137 ; RRID:Addgene_15137) -
For your References section:
Two homeobox genes define the domain of EphA3 expression in the developing chick retina. Schulte D, Cepko CL. Development. 2000 Dec . 127(23):5033-45. PubMed 11060230