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pLIA mNIC myc (CC#341)
(Plasmid #15131)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIA
  • Backbone manufacturer
    Cepko Lab
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch1 (C-term)
  • Alt name
    Notch 1
  • Species
    M. musculus (mouse)
  • Mutation
    Cytoplasmic domain initiating at amino acid 1753. See author's map and article for more information.
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIA mNIC myc (CC#341) was a gift from Connie Cepko (Addgene plasmid # 15131 ; http://n2t.net/addgene:15131 ; RRID:Addgene_15131)

  • For your References section:

    The expression and function of Notch pathway genes in the developing rat eye. Bao ZZ, Cepko CL. J Neurosci. 1997 Feb 15. 17(4):1425-34. PubMed 9006984
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