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Addgene

pLIA CRX (CC#69)
(Plasmid #15128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIA
  • Backbone manufacturer
    Cepko Lab
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CRX
  • Alt name
    cone-rod homeobox containing gene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600
  • Entrez Gene
    Crx (a.k.a. Crx1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV/SnaBI (destroyed during cloning)
  • 3′ cloning site HincII/SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An EcoRV-HincII Crx cDNA fragment containing the entire Crx coding region was subcloned into the SnaBI site of the pLIA retrovirus vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIA CRX (CC#69) was a gift from Connie Cepko (Addgene plasmid # 15128 ; http://n2t.net/addgene:15128 ; RRID:Addgene_15128)

  • For your References section:

    Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. Furukawa T, Morrow EM, Cepko CL. Cell. 1997 Nov 14. 91(4):531-41. 10.1016/S0092-8674(00)80439-0 PubMed 9390562
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