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Addgene

pTBNde(min)
(Plasmid #15125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II KS
  • Backbone size w/o insert (bp) 2960
  • Modifications to backbone
    modified to include an alpha-globin-fibronectin EDB minigene in which transcription is driven from the minimal α-globin promoter and SV40 enhancer
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-globin EDB fibronectin minigene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3151
  • Entrez Gene
    FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
  • Promoter alpha-globin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 DIR
  • 3′ sequencing primer M13 REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Hybrid minigene used for splicing assay for pre-mRNA processing. Contains a unique NdeI site for subcloning sequence of interest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTBNde(min) was a gift from Franco Pagani (Addgene plasmid # 15125 ; http://n2t.net/addgene:15125 ; RRID:Addgene_15125)

  • For your References section:

    New type of disease causing mutations: the example of the composite exonic regulatory elements of splicing in CFTR exon 12. Pagani F, Stuani C, Tzetis M, Kanavakis E, Efthymiadou A, Doudounakis S, Casals T, Baralle FE. Hum Mol Genet. 2003 May 15. 12(10):1111-20. 10.1093/hmg/ddg131 PubMed 12719375
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