Skip to main content
Addgene

L1-Fc
(Plasmid #15124)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIg-Tail
  • Backbone manufacturer
    R&D Systems
  • Backbone size w/o insert (bp) 5430
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    MC1061/P3 cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    L1-Fc
  • Alt name
    L1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3340
  • Mutation
    Chimeric molecule consisting of the Fc region of human IgG and the whole extracellular domain of human L1
  • Entrez Gene
    L1CAM (a.k.a. CAML1, CD171, HSAS, HSAS1, HYCX, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L1-Fc was a gift from Vance Lemmon (Addgene plasmid # 15124 ; http://n2t.net/addgene:15124 ; RRID:Addgene_15124)

  • For your References section:

    L1 knockout mice show dilated ventricles, vermis hypoplasia and impaired exploration patterns. Fransen E, D'Hooge R, Van Camp G, Verhoye M, Sijbers J, Reyniers E, Soriano P, Kamiguchi H, Willemsen R, Koekkoek SK, De Zeeuw CI, De Deyn PP, Van der Linden A, Lemmon V, Kooy RF, Willems PJ. Hum Mol Genet. 1998 Jun . 7(6):999-1009. 10.1093/hmg/7.6.999 PubMed 9580664
Related items:
Commonly requested with: