Pdx1-RFP hygro (DM#236)
(Plasmid
#15110)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript KS II
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePdx1 promoter
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Alt namePdx1
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Alt namepGDRF-floxhy
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SpeciesM. musculus (mouse)
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Entrez GenePdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
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Tag
/ Fusion Protein
- RFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid has floxed hygromycin for selection. Linearize with BssHII.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pdx1-RFP hygro (DM#236) was a gift from Douglas Melton (Addgene plasmid # 15110 ; http://n2t.net/addgene:15110 ; RRID:Addgene_15110) -
For your References section:
Insulin staining of ES cell progeny from insulin uptake. Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA. Science. 2003 Jan 17. 299(5605):363. 10.1126/science.1077838 PubMed 12532008
Map uploaded by the depositor.
