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M23 pEnP1 (engrailed promoter-luciferase)
(Plasmid #15103)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15103 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engrailed-2 promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2700
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primers 5′-GGAGATCTCTGGAAGTCTCCATA-3′ and 5′-CACACACACACTCTCTCCGGG-3′ were used to PCR amplify a 2.7 kb engrailed-2 promoter fragment. The fragment was cloned into pGL3B.

Contains three consensus LEF/TCF (CTTTGA/TA/T) binding sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M23 pEnP1 (engrailed promoter-luciferase) was a gift from Randall Moon (Addgene plasmid # 15103)

  • For your References section:

    Direct regulation of the Xenopus engrailed-2 promoter by the Wnt signaling pathway, and a molecular screen for Wnt-responsive genes, confirm a role for Wnt signaling during neural patterning in Xenopus. McGrew LL, Takemaru K, Bates R, Moon RT. Mech Dev. 1999 Sep . 87(1-2):21-32. 10.1016/S0925-4773(99)00136-7 PubMed 10495268
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