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Addgene

V45 pHIPPY PGL3 Luciferase
(Plasmid #15099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIPPY
  • Backbone manufacturer
    Moon Lab
  • Backbone size w/o insert (bp) 2167
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGL3 luciferase siRNA
  • Insert Size (bp)
    19

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer H1 primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs

See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case.

Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V45 pHIPPY PGL3 Luciferase was a gift from Randall Moon (Addgene plasmid # 15099 ; http://n2t.net/addgene:15099 ; RRID:Addgene_15099)

  • For your References section:

    A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. Kaykas A, Moon RT. BMC Cell Biol. 2004 Apr 30. 5():16. 10.1186/1471-2121-5-16 PubMed 15119963
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