V44 pHIPPY blunt
(Plasmid
#15098)
-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepHIPPY
-
Backbone manufacturerMoon Lab
- Backbone size (bp) 2167
-
Vector typeMammalian Expression, RNAi
-
Selectable markersZeocin
Growth in Bacteria
-
Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer H1 primer (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
V44 pHIPPY blunt was a gift from Randall Moon (Addgene plasmid # 15098 ; http://n2t.net/addgene:15098 ; RRID:Addgene_15098) -
For your References section:
A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. Kaykas A, Moon RT. BMC Cell Biol. 2004 Apr 30. 5():16. 10.1186/1471-2121-5-16 PubMed 15119963
Map uploaded by the depositor.
