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Addgene

NFkBm2
(Plasmid #15090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-146a promoter
  • Alt name
    microRNA miR-146a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    547
  • Mutation
    2 NFkB binding sites mutated

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Two NFkappaB binding sites are mutated: GGGATTTCCC to accATTTCCC; GGGACTTTCC to accACTTTCC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NFkBm2 was a gift from David Baltimore (Addgene plasmid # 15090 ; http://n2t.net/addgene:15090 ; RRID:Addgene_15090)

  • For your References section:

    NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Taganov KD, Boldin MP, Chang KJ, Baltimore D. Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6. 10.1073/pnas.0605298103 PubMed 16885212
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